Calcium Phosphate Based Three-Dimensional Cold Plotted Bone Scaffolds for Critical Size Bone Defects

Figure 3

DNA content (crystal violet staining) and extracellular matrix production levels (alcian blue staining) of ATDC5 cells in preconditioned proliferation and differentiation medium. The absorbance of the extracted crystal violet and alcian blue was used to calculate the DNA content and extracellular matrix production relative to the absorbance value at day 0. (a) DNA content during proliferation conditions. (b) DNA content during differentiation conditions. (c) Extracellular matrix production during proliferation conditions. (d) Extracellular matrix production during differentiation conditions.