Potential Biomarkers and Their Applications for Rapid and Reliable Detection of Malaria

Figure 6

The immunocapture Plasmodium lactate dehydrogenase (ICpLDH) assay. A schematic of the reaction is shown in which the pLDH is immobilized using a monoclonal antibody. The enzyme activity can be measured using a coupled enzyme assay that generates APADH. The latter reduces nitro blue tetrazolium, a chromogenic substrate, using an enzyme diaphorase. Activity is quantified spectrophotometrically at 650 nm and plotted as a function of percentage parasitemia [118].