Research Article
Molybdenum Reduction to Molybdenum Blue in Serratia sp. Strain DRY5 Is Catalyzed by a Novel Molybdenum-Reducing Enzyme
Table 1
Partial purification scheme of Mo-reducing enzyme from Serratia sp. strain DRY5.
| | Fraction | Total protein (mg) | Specific activity (units/mg protein) | Total activity (units) | Yield % | Fold purification |
| | Crude | 500 | 3 | 1500 | 100.0 | 1.0 | | Mono Q | 120 | 11.5 | 1380 | 92 | 3.8 | | Mono Q* | 10 | 17.5 | 175 | 11.66 | 5.8 | | Zorbax GF-250 | 1.2 | 24.3 | 29.16 | 1.9 | 8.1 | | Chromatofocusing | 0.1 | 0.25 | 0.25 | 0.0002 | 0.08 |
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