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BioMed Research International
Volume 2014 (2014), Article ID 854954, 9 pages
http://dx.doi.org/10.1155/2014/854954
Research Article

Therapeutic Effects of PADRE-BAFF Autovaccine on Rat Adjuvant Arthritis

1State Key Laboratory of Cancer Biology, Department of Neurology, Xijing Hospital, Fourth Military Medical University, 17 Changle West Road, Shaanxi, Xi’an 710032, China
2State Key Laboratory of Cancer Biology, Department of Biopharmaceutics, School of Pharmacy, Fourth Military Medical University, 17 Changle West Road, Xi’an 710032, China
3The Biotechnology Center, Fourth Military Medical University, 17 Changle West Road, Xi’an, Shaanxi 710032, China
4Key Laboratory of Biomedical Information Engineering of the Ministry of Education, Department of Biological Science and Engineering, School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710049, China
5Department of Anesthesiology, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA

Received 5 November 2013; Revised 17 February 2014; Accepted 19 February 2014; Published 25 March 2014

Academic Editor: Thomas Griffith

Copyright © 2014 Guo-dong Feng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

B cell activating factor (BAFF) is a cytokine of tumor necrosis factor family mainly produced by monocytes and dendritic cells. BAFF can regulate the proliferation, differentiation, and survival of B lymphocytes by binding with BAFF-R on B cell membrane. Accumulating evidences showed that BAFF played crucial roles and was overexpressed in various autoimmune diseases such as systemic lupus erythematous (SLE) and rheumatoid arthritis (RA). This suggests that BAFF may be a therapeutic target for these diseases. In the present study, we developed a BAFF therapeutic vaccine by coupling a T helper cell epitope AKFVAAWTLKAA (PADRE) to the N terminus of BAFF extracellular domains (PADRE-BAFF) and expressed this fusion protein in Escherichia coli. The purified vaccine can induce high titer of neutralizing BAFF antibodies and ameliorate the syndrome of complete Freund’s adjuvant (CFA) induced rheumatoid arthritis in rats. Our data indicated that the BAFF autovaccine may be a useful candidate for the treatment of some autoimmune diseases associated with high level of BAFF.