Role of Plasma Membrane Caveolae/Lipid Rafts in VEGF-Induced Redox Signaling in Human Leukemia Cells
Figure 5
Effect of different inhibitors of VEGF/VEGFR-2 interaction on intracellular ROS level in B1647 cells. Cells, control, and serum starved (−HS, “Control dep”) were treated with 500 μg/mL Bevacizumab for 30 min or 5 μM Cav-1 scaffolding domain (CSD) for 2 h, in the presence or absence of 50 ng/mL VEGF; then, the intracellular ROS level was spectrofluorimetrically measured by means of DCFH-DA, as described in Section 2. *: significantly different from control cells; : significantly different from serum deprived control cells (−HS).
