Role of Plasma Membrane Caveolae/Lipid Rafts in VEGF-Induced Redox Signaling in Human Leukemia Cells
Figure 7
Caveolin-1 interaction with Nox2 and VEGFR-2 in the presence or absence of VEGF in B1647 cells. Cells deprived or not of serum (±HS) were subjected to immunoprecipitation with anti-caveolin-1 as described in Section 2. (a) Samples were electrophoresed, immunoblotted, and revealed for anti-Nox2 and anti-caveolin-1; a representative blot is shown. (b) Densitometric analysis normalized for caveolin-1 content and expressed as fold change in Nox2 expression with respect to control (+HS). (c) Samples were electrophoresed, immunoblotted, and revealed for anti-VEGFR-2 and anti-caveolin-1; a representative blot is shown. (d) Densitometric analysis normalized for caveolin-1 content and expressed as fold change in VEGFR-2 expression with respect to control (+HS). Results were obtained considering three independent Western blot experiments. *: significantly different from control cells (+HS); °: significantly different from control serum starved cells (−HS).
