Conditioned Media from Microvascular Endothelial Cells Cultured in Simulated Microgravity Inhibit Osteoblast Activity

Figure 1

Simulated microgravity inhibits HMEC growth. (a) HMEC were cultured for different times in the RWV (HMEC-RWV) and trypsinized and viable cells were counted. HMEC-C: control. (b) Cell extracts (50 μg/lane) were loaded on a 15% SDS-PAGE, blotted into nitrocellulose filter, incubated with anti-p21 and anti-p53 antibodies, and visualized by chemiluminescence as described. After stripping, the blot was incubated with an anti-GAPDH antibody to show that comparable amounts of proteins were loaded per lane. (c) Apoptosis was evaluated by ELISA on HMEC lysates after 48 and 72 h in the RWV or under control conditions. Our positive control is represented by HMEC exposed to H₂O₂ for 30 min and then cultured for additional 48 h.

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