Conditioned Media from Microvascular Endothelial Cells Cultured in
Simulated Microgravity Inhibit Osteoblast Activity
Figure 6
CM from HMEC in simulated microgravity inhibit Saos-2 activity. Saos-2 were cultured for 7 and 14 days with CM from HMEC in simulated microgravity (CM-RWV) or by HMEC controls (CM-C) both added with osteogenic stimuli. (a) ALP enzymatic activity and (b) Alizarin Red Staining were performed as above.