Assessment of Tumor Cells in a Mouse Model of Diffuse Infiltrative Glioma by Raman Spectroscopy
Figure 2
Raman spectrum of infiltrative glioma in the mouse brains. (a) The average spectra in the six brains at 1030 (C–H; phenylalanine), 1050–1100 (C–C; lipid), 1100 (O–P–O; DNA), and 1200–1300 cm−1 (amide III) were significantly higher in glioma tissues (red) than in the normal brains (blue). (b) Statistical analysis. Statistical significance of the difference between tumor and normal samples was measured for each spectrum using the value (red) and false discovery rate (FDR; blue) based on t-statistics (FDR was computed by permutation label permutation). (c) Representative images of H&E staining and 3D plots of principal component analysis. The clusters of tumor (red ellipsoids) were separated from those of normal (blue ellipsoids), which supported the significance of spectral difference between tumor tissues and normal tissues.
