Research Article

PCR and Microscopic Identification of Isolated Leishmania tropica from Clinical Samples of Cutaneous Leishmaniasis in Human Population of Kohat Region in Khyber Pakhtunkhwa

Table 2

Days detected for observation of promastigotes in the culture media of RPMI-1640 from suspected lesions.

Media1st day2nd day3rd day4th day5th day6th day7th day8th day9th day10th day11th day12th day

RPMI 1640++++++++++++
Negative control

(−) indicates amastigote.
(+) indicates promastigotes.
(++) indicates promastigotes at peak level.