CELLCOUNTER: Novel Open-Source Software for Counting Cell Migration and Invasion In Vitro

Figure 1

The graphical user interface of CELLCOUNTER. Green circles indicate automatically recognized cells and red circles indicate manually labeled cells. Functions for buttons/inputs are (1) manual counting/curation; (2) zooming in; (3) zooming out; (4) restoring to the original image size; (5) saving the counting results (including images); (6) setting the radius of the label (for visualization only); (7) setting the radius of small wells (in px, see the main text for more details, default recommended).