CELLCOUNTER: Novel Open-Source Software for Counting Cell Migration and Invasion In Vitro

Table 1

Images used in this study. Cells were stained with 0.1% crystal violet for 20 minutes for the deep staining group and 8 minutes for the light staining group. High density images refer to those having over 400 cells and low density images refer to those having less than 200 cells. Biology stimulation of cells was discussed in Section 2.

ID	Cell line	Stimulation	Stain	Density	Number of cells
					Expert	CELLCOUNTER	Beginner
1	T47D	No	—	High	931	957	955
2	MCF-7	No	Deep	High	792	748	538
3	MDA-MB-231	No	—	High	475	479	413
4	pc-3	No	—	—	523	507	390
5	MCF-7	—	Deep	—	314	322	263
6	MCF-7	Yes	Deep	—	359	364	300
7	MDA-MB-231	Yes	—	High	566	572	366
8	Hep3B	—	Light	—	284	279	249
9	MDA-MB-435s	—	—	—	464	461	430
10	Hep3B	—	—	—	186	178	144
11	MCF-7	—	Light	Low	165	159	—
12	MCF-7	Yes	—	—	403	403	—
13	MCF-7	No	Deep	—	417	395	—
14	MCF-7	Yes	Light	—	189	187	—
15	MCF-7	—	Light	Low	94	98	—
16	MCF-7	Yes	—	Low	120	118	—
17	MCF-7	—	—	Low	139	140	—
18	MCF-7	—	Light	—	8	8	—
19	MCF-7	—	Deep	High	554	553	—
20	MCF-7	—	—	Low	191	192	—