GABAB Receptors Expressed in Human Aortic Endothelial Cells Mediate Intracellular Calcium Concentration Regulation and Endothelial Nitric Oxide Synthase Translocation
Figure 3
GABAB receptors regulate [Ca2+]i in HAECs. (a) 100 μM baclofen caused increase of [Ca2+]i in the cultured HAECs. (b) Pretreatment with 1 mM CGP46381 partly (~50%) abolished the increase of [Ca2+]i induced by 100 μM baclofen. (c) Pretreatment with 1 mM CGP55845 completely inhibited the increase of [Ca2+]i induced by 100 μM baclofen. (A) Representative images acquired at 5 s, 38.94 s, and 148.96 s, respectively. (B) Fluorescent intensity in the images acquired before and after baclofen application. The red bar represents the time when baclofen was added. FI represents the fluorescence intensity color scale with the direction of the arrow indicating higher intensity.
