Modulation of c-Fos and BDNF Protein Expression in Pentylenetetrazole-Kindled Mice following the Treatment with Novel Antiepileptic Compound HHL-6
Figure 3
Effect of HHL-6 on scPTZ-induced kindling. The kindling scores are expressed as the arithmetic mean ± SEM (/group). The treatment of 20 mg/kg of HHL-6 showed a slight increase in their seizure score from treatment day 9 to 13 and thereafter it returned to the 0. The bar under the figure represents the timeline of the experiments. The mice were treated with PTZ or saline on alternate days. A total of 18 doses were given during the 36-day period when animals in control group were fully kindled. The average scores of PTZ-induced seizures were significantly higher in the kindled control mice (receiving no other treatment except for PTZ). *: significantly higher than normal control revealed by nonparametric Mann-Whitney tests. The animals were sacrificed for immunohistochemistry and RT-PCR analysis after 36 days.
