Prooxidant Effects of Verbascoside, a Bioactive Compound from Olive Oil Mill Wastewater, on In Vitro Developmental Potential of Ovine Prepubertal Oocytes and Bioenergetic/Oxidative Stress Parameters of Fresh and Vitrified Oocytes

Table 2

Effects of verbascoside on cleavage and blastocyst developmental rates of prepubertal lamb oocytes after IVF and IVP.

Verbascoside concentration (𝜇M)	Number of MII oocytes	Number of cleaved embryos (%)		Number of blastocysts (%)
		24 h after-IVF	30 h after-IVF	At day 7	At day 8
0	200	76 (38)	150 (75)	28 (18.7)^a	36 (24)^a
1.03	240	103 (42.9)	189 (78.7)	38 (20.1)	59 (31.2)
2.06	220	77 (35)	156 (70.9)	18 (11.5)	18 (11.5)^b
4.11	240	93 (38.7)	185 (77.1)	11 (5.9)^b	22 (11.9)^b

Chi-square test: ^a,b𝑃<0.05.