Delivery of Human EV71 Receptors by Adeno-Associated Virus Increases EV71 Infection-Induced Local Inflammation in Adult Mice
Figure 1
Expression of recombinant AAV-hSCARB2 and AAV-hPSGL1 in cell lines increased susceptibility to EV71 infection. (a) Green fluorescence protein (GFP), human PSGL1 (hPSGL1), and human SCARB2 (hSCARB2) were cloned into the pEMBL plasmid with a CB promoter (pEMBL-CB). (b) L929 cells were transfected with pEMBL-CB-hPSGL1 and the expression of hPSGL1 was detected by surface stained with anti-hPSGL1 antibody and FITC-conjugate anti-mouse IgG and then analyzed by flow cytometry. (c) L929 cells were transfected with pEMBL-CB-hSCARB2 and the expression of hSCARB2 was detected by surface stained with anti-human SCARB2 and FITC-conjugate anti-mouse IgG and then analyzed by flow cytometry. (d) L929 cells transfected with the vector pEMBL-CB (Mock), pEMBL-CB-hSCARB2 (hSCARB2), and pEMBL-CB-hPSGL1 (hPSGL1) were infected with live EV71 (MOI = 1.0). Forty-eight hours after infection, protein extracted from cells was analyzed by Western blot using anti-EV71 VP1 and anti-actin antibodies. (e) Identical L929 cell treatment as described in (d) was observed for the cytopathic effect (CPE) by microscopy. Cells with CPE are labeled with a black arrow.
