Delivery of Human EV71 Receptors by Adeno-Associated Virus Increases EV71 Infection-Induced Local Inflammation in Adult Mice
Figure 3
Expression of hSCARB2 and hPSGL1 in mice after rAAV delivery. ICR mice were administered 5 × 1011 vg of either rAAV-hSCARB2 or rAAV-hPSGL1 by the i.v. and oral routes. After three weeks, the mice were sacrificed and the organs, including the brain, heart, lung, spleen, intestine, colon, kidney, blood, and liver, were collected; RNA was extracted for real-time RT-PCR analysis (a). All of the data shown represent one of the three mice. Same organs were also collected from untreated ICR mice as control. The brain and intestines were also collected for frozen section and immunohistochemistry analysis. Expression of hSCARB2 and hPSGL1 was detected using the anti-hSCARB2 and anti-hPSGL1 antibodies, respectively (b). Positive cells are labeled with a black arrow.
