Delivery of Human EV71 Receptors by Adeno-Associated Virus Increases EV71 Infection-Induced Local Inflammation in Adult Mice
Figure 5
Upregulation of cytokines or chemokines in brain tissue in rAAV-hSCARB2- and rAAV-hPSGL1-transduced mice. Adult ICR mice were i.v. injected and orally administered 100 μL of 5 × 1012 vg/mL of rAAV-hSCARB2 (a) or rAAV-hPSGL1 (b) and then infected with EV71 (1 × 107 pfu in 100 L) three weeks later via i.p. injection. Seventy-two hours after EV71 infection, RNA was extracted from the brain tissue and analyzed by real-time RT-PCR to determine the expression of cytokines (IL-1, IL-6, IFN, and TNF), chemokines (CCR2, IP-10, MCP-1, and MIP-1), and GAPDH. RNA was also extracted from untreated mice as control. The related gene expression was calculated using the comparative method for the relative quantity normalized to GAPDH gene expression. , , and (-test). All of the samples were collected from three individual animals ().
