Tracking the Biogenesis and Inheritance of Subpellicular Microtubule in Trypanosoma brucei with Inducible YFP-α-Tubulin
Figure 1
Inducible expression of YFP-α-tubulin in T. brucei. Cells stably transfected with pLew-YFP-α-tubulin were cultivated in the absence or presence of tetracycline to induce YFP-α-tubulin expression. Samples were taken at various time points for immunoblots (a), growth curve analyses (b), and cell fractionation studies (c). YFP-α-tubulin was detected as early as 2 hours after induction. Continuous induction led to slightly increased YFP-α-tubulin level and had little effect on parasite proliferation. Immunoblots of detergent extracted YFP-α-tubulin cells indicated that only a small amount of YFP-α-tubulin was incorporated into the detergent insoluble cytoskeleton (P). T: total cells; S: detergent soluble fraction.
