Percentage of HEp-2 cells detachment after 5 h of incubation with the pet+ aEPEC strains BA 3160; BA 2991; BA 2923; BA 2775 and the EAEC strain 215 A3 in the presence of DMEM-tryptone. Positive control EAEC 042. Negative controls just the cells (C) and pet− aEPEC strain BA 3170. The data refer to the mean values of four independent experiments. Significantly different when compared to the negative control (C). One-way analysis of variance (ANOVA) with Tukey’s test was carried out to determine statistically significant differences from untreated controls. .
