Pet is present in the HEp-2 culture supernatant after 5 h of incubation in DMEM-tryptone with the aEPEC strains BA 3160, BA 2923, BA 2775, and BA 2991, as detected with anti-Pet IgG and developed with DAB. MW: molecular weight. Positive controls: EAEC 042 and Pet. Negative controls: aEPEC BA 3170 and noninfected cells (NC).
