Synthesis and Gene Silencing Properties of siRNAs Containing Terminal Amide Linkages

Figure 6

RNAi activity of native and modified siRNAs toward luciferase gene expression in HeLa cells. Small interfering RNAs were transfected at 10 nM concentration and luciferase activities were determined after 48 h. The firefly luciferase activity (Luc) was then normalized to that of Renilla luciferase (Rl) and the uninhibited activity of cells transfected with an unrelated siRNA (nc) was set to 1. Data are the mean ± s.d. from at least three experiments.