Acute and Subacute Toxicity In Vivo of Thermal-Sprayed Silver Containing Hydroxyapatite Coating in Rat Tibia

Table 2

Laboratory parameters in the subacute phase (4–12 weeks postoperatively).

Time after treatment	Implant coating	GOT (IU/L)	GPT (IU/L)	LDH (IU/L)	Creatinine (mg/dL)	BUN (mg/dL)
4 weeks	Control; (14-week-old)	72.7±7.1	34.0±2.6	820.3±267.8	0.25±0.02	20.8±0.4
	HA	95.6±12.9^∗	31.6±1.2	1180.3±475.6	0.27±0.01	22.1±1.4
	2% AgHA	82.7±5.1	34.7±1.2	884.7±163.1	0.25±0.02	22.3±0.8
	50% AgHA	73.7±7.5	34.3±2.1	619.3±332.6	0.27±0.02	21.3±1.2
8 weeks	Control; (18-week-old)	80.4±37.6	35.6±5.0	836.2±904.7	0.28±0.05	21.6±2.5
	HA	131.0±20.1^∗∗	38.2±3.4	2202.2±824.6^∗∗∗	0.31±0.03	23.2±4.2
	2% AgHA	102.0±5.1	40.6±5.2	1562.8±278.4	0.33±0.02	23.5±1.9
	50% AgHA	78.4±14.8	36.8±2.7	705.2±304.6	0.31±0.01	23.5±1.7
12 weeks	Control; (22-week-old)	99.7±8.1	33.3±2.3	1649.0±306.2	0.31±0.03	19.6±2.1
	HA	90.5±9.2	33.5±4.9	1799.0±429.9	0.31±0.01	20.3±2.2
	2% AgHA	101.8±12.5	36.0±3.4	1895.0±527.2	0.31±0.01	22.2±1.0
	50% AgHA	82.3±2.5	32.3±2.1	1367.7±157.1	0.28±0.01	21.7±1.5

Five 14-, 18-, and 22-week-old male Sprague-Dawley rats with no treatment were used as controls for 4, 8, and 12 weeks, respectively. *Mean GOT levels at 4 weeks differed significantly between controls (14-week-old rats) and the HA group (𝑃<0.05). ** and ***are the mean GOT and LDH levels at 8 weeks that differed significantly between controls (18-week-old rats) and the HA group (𝑃<0.05).
GOT: glutamic-oxaloacetic transaminase; GPT: glutamic-pyruvic transaminase; LDH: lactate dehydrogenase; BUN: blood urea nitrogen.