Combined Cytolytic Effects of a Vaccinia Virus Encoding a Single Chain Trimer of MHC-I with a Tax-Epitope and Tax-Specific CTLs on HTLV-I-Infected Cells in a Rat Model
Figure 5
Combined effects of 4O1/C8 and m8Δ on killing of CTL-resistant HTLV-I-infected cells. (a) FPM1V.EFGFP/8R cells were exposed to m8Δ/RT1AlSCTax180L, m8Δ/RT1AlSCNLEnv371L, or PBS for 2 hrs at indicated MOI. After extensive wash, the cells were cocultivated with 4O1/C8 for indicated periods and subjected to flow-cytometric analysis for the expression of EGFP at the indicated days. Percentage of EGFP positive cells is indicated in each panel. (b) Bar graph of the flow-cytometric data. The data are presented as mean ± SD of triplicate wells. Asterisks indicate statistical significance () compared to the m8Δ/RT1AlSCNLEnv371L-infected cells with corresponding MOI.