Induction of Antibodies and T Cell Responses by a Recombinant Influenza Virus Carrying an HIV-1 Tat Protein in Mice
Figure 1
Construction and expression of WSN/Tat virus. The coding sequence of the Tat protein of HIV IIIB (BH-10 clone), that lacked residues 51 to 59 of the basic region, was inserted into the cloning cassette of the plasmid pPolI-WSN HA, after the HA signal peptide (SP). CT, TM = cytoplasmic, transmembrane domains of HA, respectively (a). Viral protein lysates were separated on a 12% polyacrylamide gel and analyzed in Western blots by using mouse monoclonal antibodies specific to the HA1 of WSN virus or a specific rabbit anti-Tat hyperimmune serum (b). Genetic stability of WSN/Tat virus was determined in four different plaques by RT-PCR after six passages on MDCK cells. bp DNA ladder (c).
