Induction of Antibodies and T Cell Responses by a Recombinant Influenza Virus Carrying an HIV-1 Tat Protein in Mice
Figure 3
In vitro and in vivo replication of WSN/Tat virus. Growth properties of WSN and WSN/Tat viruses in MDCK cells. The viruses were used to inoculate MDCK cells at a multiplicity of infection of 0.01, and at the indicated times after infection virus titers in the supernatants were determined (a). Three groups of BALB/c mice (/group) were i.n. infected with 103 PFU of WSN virus and three other groups of mice (/group) with 105 PFU of WSN/Tat virus. Animals from each group were euthanized on days 1, 3, and 5 p.i., and lungs were collected from individual mice for viral titration on MDCK cells (b). Progesterone-treated mice were i.vag. infected with the above viruses, and viral titers in vaginal washes from individual mice were determined at the indicated time points by endpoint titration in MDCK cells (c). Bars represent means ± standard deviation (SD) for four mice per group.
