Induction of Antibodies and T Cell Responses by a Recombinant Influenza Virus Carrying an HIV-1 Tat Protein in Mice
Figure 4
Tat-specific T cell responses in mice vaccinated with WSN/Tat virus. BALB/c mice (/group) were immunized i.n. or i.vag. with WSN/Tat virus and 7 days later Tat-specific T cell responses were measured in cells from distinct pools of draining lymph nodes, MLN and ILN, respectively, and freshly isolated splenocytes by use of an ex vivo IFN- ELISPOT assay with the indicated peptides (a). Splenocytes from mice immunized with WSN/Tat or WSN virus were cultured for 5 days in the presence of VCF and then washed for the IFN- ELISPOT assay. The values reported are those obtained from stimulated cells minus the background from nonstimulated cells. Bars represent the mean values ± SD of triplicate wells. The data are representative of three independent experiments that gave similar results (b). Splenocytes from the above mice were restimulated in the presence of different concentrations of Tat protein for 5 days and the resulting proliferation was assessed by measuring [3H] thymidine incorporation. Results are expressed as the ratio between values (averages of quintuplicates from stimulated and nonstimulated cultures). SI = stimulation index (c).
