Research Article
Crystallographic Analysis of Ground and Space Thermostable T1 Lipase Crystal Obtained via Counter Diffusion Method Approach
Table 2
Final statistics of refined structure for the ground-grown and space-grown T1 lipase crystals.
| | Ground-grown crystal | Space-grown crystal |
| | Resolution range used in refinement (Å) | 30–1.2 | 30–1.2 | | Number of reflections used in refinement | 272755 | 271607 | | R factor | 0.134 | 0.129 | | R free | 0.162 | 0.150 | | Number of protein atoms | 387 | 387 | | Number of water molecules | 721 | 781 | | Number of metal ions | 7 | 7 | | Number of glycerols | 4 | 4 | | RMS deviation | | | | Bond length (Å) | 0.021 | 0.020 | | Angles (Deg) | 1.882 | 1.822 | | Planes (Å) | 0.014 | 0.014 | | Ramachandran plot details | | | | Residues in favoured regions (%) | 91.5 | 91.8 | | Residues in additional allowed regions (%) | 7.9 | 7.6 | | Residues in generously allowed regions (%) | 0.3 | 0.3 | | Residues in disallowed regions (%) | 0.3 | 0.3 | | Average B-factor value (A
2) | | | | Chain A | 10.8 | 9.4 | | Chain B | 12.1 | 10.5 |
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