Cytoskeleton Modifications and Autophagy Induction in TCam-2 Seminoma Cells Exposed to Simulated Microgravity
Figure 6
Autophagy induction in TCam-2 cells exposed to microgravity. (a) Immunodetection of LC3 in TCam-2 cells cultured for 24 hours at 1 g (II) or under RPM (IV) conditions. In VI LC3 immunodetection of TCam-2 cells cultured in RPM condition for 48 hours is reported. In I, III, and V the respective bright fields are shown. (b) Stack profile of 24 hours of culture representative experiment showing the maximum amplitude (MAX Amplitude) of fluorescence in 12 regions of interest (ROI), randomly drawn in an area in which the cells reached confluence, in nonrotated (I) and RPM cultured samples (II). It is evident an increase of maximum amplitude of fluorescence in microgravity exposed samples (II) with respect to the 1 g-cultured cells (I). (c) MEAN (A) ( versus 14.34 ± 0.59; ) and SUM (I) ( versus 6.95 ± 1.52; ) confirm an increase of LC3 positivity in RPM exposed sample with respect to 1 g cells after 24 hours of culture. Data are expressed as the mean ± SEM.
