Successive Nonstatistical and Statistical Approaches for the Improved Antibiotic Activity of Rare Actinomycete Nonomuraea sp. JAJ18

Table 7

Experimental validation of the combined effect of variables under optimized and unoptimized conditions on the antibiotic activity of Nonomuraea sp. JAJ18.

Variables	Level (g/L)		Antibiotic activity (U/mL)
	Unoptimized	Optimized	Unoptimized	Optimized (predicted)	Optimized (experimental)
Starch	10	15.6	140.0	154.49	161.7
NaCl	5	0.8
MgSO4·7H2O	1	1.98