A Novel Mutation in Leptin Gene Is Associated with Severe Obesity in Chinese Individuals

Table 2

Primer Sequences for the amplification.

Name	Forward primer (5′–3′)	Reverse primer (5′–3′)	Product length (bp)	Annealing temperature (°C)
Primer 1	GCCAGAGCAGAAAGCAAA	TCAGGAGGCGTTCAATAA	397	59
Primer 2	GAGCACTTGTTCTCCCTCTT	TTCCCTTAACGTAGTCCTTG	435	61