Involvement of p53 Mutation and Mismatch Repair Proteins Dysregulation in NNK-Induced Malignant Transformation of Human Bronchial Epithelial Cells
Figure 1
Malignant transformation of NHBE cells with NNK. (a) Eight weeks after NNK exposure, MTT assay was performed to measure cell proliferation rate. , , compared with NHBE cells. Values are the means ± SD of at least three independent experiments. (b) NHBE and NHBE-NNK8 cells were assayed for anchorage-independent growth in soft agar. Representative cell colonies are shown. (c) Quantitative analysis of colony numbers of (b). Values are the means ± SD of at least three independent experiments. . (d) Analysis of cell migration in NHBE and NHBE-NNK8 cells by wound-healing assay. Representative photographs were taken at 0 h, 24 h, and 48 h after wound. Magnification, ×40. (e) The wound closure of (d) was quantified by measuring the remaining unmigrated area. Values are the means ± SD of at least three independent experiments. , .
