Involvement of p53 Mutation and Mismatch Repair Proteins Dysregulation in NNK-Induced Malignant Transformation of Human Bronchial Epithelial Cells
Figure 5
Restoration of wild-type p53 expression inhibited the proliferation of malignant transformed NHBE-NNK8 cells. (a) MTT assay was performed to measure cell proliferation rate. , , compared to NHBE-NNK8 and NHBE cells. , compared to p53 transfected NHBE-NNK8 and NHBE-NNK8 cells. Values are the means ± SD of at least three independent experiments. (b) Western blot analysis of endogenous and ectopically expressed p53 in NHBE, NHBE-NNK8, and p53 transfected NHBE-NNK8 cells. GAPDH was detected as loading control. (c) DNA contents of NHBE-NNK8 and p53 transfected NHBE-NNK8 cells were analyzed by flow cytometry and FlowJo software. Numbers indicate the percentages of cells in the different phases of the cell cycle. Data are representative results from three independent experiments. PI, propidium iodide.
