Research Article
Preliminary Characterization of Mitochondrial Genome of Melipona scutellaris, a Brazilian Stingless Bee
Table 1
Annotated genes in M. scutellaris mitogenome, upstream/downstream relations, and similarity analysis.
| Reference gene | Upstream gene | Downstream gene | Similarity to reference sequence | Name | OCR* | Name | OCR* | Name | OCR* | Blast algorithm | Best identity | E-value |
| trnI | 2 | trnA | 2 | trnQ | 2 | blastn | — | — |
| trnA | 34 | trnT trnN | 27 25 | rrnS | 30 | blastn | 92.06 | 2.00E − 021 |
| trnK | 16 | trnQ | 5 | trnE trnM | 6 4 | blastn | 93.62 | 2.00E − 016 |
| trnM | 28 | trnX | 22 | nad2 | 27 | blastn | 95.71 | 2.00E − 028 |
| nad2 | 36 | trnM | 27 | trnC | 32 | tblastx | 96.43 | 2.00E − 018 |
| trnC | 32 | nad2 | 32 | trnW | 30 | blastn | — | — |
| trnW | 32 | trnC | 30 | trnY | 25 | blastn | 96.92 | 6.00E − 028 |
| trnY | 26 | trnW | 25 | cox1 | 25 | blastn | — | — |
| cox1 | 36 | trnY | 25 | trnL2 | 20 | tblastx | 98.33 | 2.00E − 099 |
| trnL2 | 20 | cox1 | 20 | cox2 | 20 | blastn | — | — |
| cox2 | 36 | tnrL2 | 20 | tnrD | 32 | tblastx | 96.55 | 3.00E − 063 |
| trnD | 32 | cox2 | 32 | atp8 | 31 | blastn | — | — |
| atp8 | 35 | trnD | 31 | atp6 | 34 | tblastx | 93.75 | 2.00E − 005 |
| atp6 | 36 | atp8 | 34 | cox3 | 36 | tblastx | 94.44 | 1.00E − 009 |
| cox3 | 36 | atp6 | 36 | trnV | 17 | tblastx | 94.12 | 4.00E − 027 |
| trnS1 | 27 | trnR nad3 | 12 10 | trnF | 19 | blastn | 96.88 | 2.00E − 027 |
| trnG | 3 | cox3 | 3 | nad3 atp8 | 2 1 | blastn | — | — |
| nad3 | 36 | trnV cox3 | 17 14 | trnR | 24 | tblastx | 95.00 | 7.00E − 006 |
| trnR | 24 | nad3 | 24 | trnS1 trnN | 12 9 | blastn | — | — |
| trnN | 34 | rrnL | 25 | trnA | 25 | blastn | 96.72 | 9.00E − 026 |
| trnE | 12 | trnK | 6 | trnF | 7 | blastn | — | — |
| trnF | 28 | trnS1 | 19 | nad5 | 15 | blastn | — | — |
| nad5 | 36 | trnF | 15 | trnQ | 35 | tblastx | 95.83 | 1.00E − 013 |
| trnH | 0 | | | | | | — | — |
| nad4 | 36 | trnQ | 35 | nad4l | 28 | tblastx | 95.00 | 1.00E − 028 |
| nad4l | 33 | nad4 | 28 | trnP | 26 | tblastx | 94.74 | 0.13 |
| trnT | 32 | nad4l | 9 |
trnA |
27 | blastn | — | — | trnQ | 7 |
| trnP | 35 | nad4l | 26 | nad6 | 35 | blastn | — | — |
| nad6 | 35 | trnP | 36 | cob | 35 | tblastx | 94.12 | 4.00E − 022 |
| cob | 36 | nad6 | 35 | trnS2 | 35 | tblastx | 95.65 | 1.00E − 011 |
| trnS2 | 35 | cob | 35 | nad1 | 35 | blastn | 100.00 | 7.00E − 032 |
| nad1 | 36 | trnS2 | 35 | rrnL | 34 | tblastx | 92.86 | 5.00E − 021 |
| trnL1 | 2 | trnY nad1 | 1 1 | rrnL trnL2 | 1 1 | blastn | — | — |
| rrnL | 36 | nad1 | 34 | trnN | 25 | blastn | 95.26 | 3.00E − 095 |
| trnV | 23 | cox3 | 17 | nad3 | 17 | blastn | — | — |
| rrnS | 36 | trnA | 30 | tnrQ | 29 | blastn | 95.08 | 4.00E − 024 |
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OCR: occurrences in different assemblies.
**Genes not found in M. bicolor mitogenome.
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