Review Article

The Impact of Simulated and Real Microgravity on Bone Cells and Mesenchymal Stem Cells

Table 1

Comparative methods of 3D cell culture systems using simulated .

DeviceWorking principle

Free fall machineFFMFree fall for 800 ms, “bounce” of 20 g for 50 ms

Levitating magnetsLMA high gradient magnetic field prevents sedimentation

2D-clinostatRotation along one axis

Random positioning machineRPMTwo frames with randomized movement

Rotating wall vesselRWVConstant rotation prevents cells from settling