Developmental Stage-Specific Regulation of the Circadian Clock by Temperature in Zebrafish
Figure 4
Acute temperature shift regulates zfper1b expression during early development. (a) Schematic representation of the experimental design. After fertilization, embryos were raised under a constant 29°C and at different developmental stages were acutely shifted for 4 h to 23.5°C (red arrows) (circa 20 embryos for each experimental group). Then mRNA from temperature shifted groups and a control group maintained at a constant 29°C were isolated (black arrows). (b) Images of embryos raised at 29°C (control) or 4 hrs after a temperature shift applied at different developmental stages. The developmental stage where the temperature shift induced zfper1b expression is indicated by a blue box. (c) Expression level of zfper1b measured in the different groups of embryos by qRT-PCR (control in black and temperature shift groups in blue).
