Comparative Analysis of Proliferation and Differentiation Potentials of Stem Cells from Inflamed Pulp of Deciduous Teeth and Stem Cells from Exfoliated Deciduous Teeth
Figure 3
SCIDs and SHEDs showed similar adipogenic differentiation potentials. (a) SCIDs and SHEDs were cultured in adipogenesis differentiation medium for 3 weeks. Cells were stained with Oil Red O solution to visualize lipid deposits before (top, 0 d) and after (bottom, 21 d) adipogenic induction. Scale bar: 20 μm. (b) CD36 expression was not significantly changed in SHEDs after adipogenic induction but significantly increased in SCIDs; however, the difference between SCIDs and SHEDs was not significant. (c–e) Real-time RT-PCR results showed similarly enhanced expression of (c) LPL, (d) PPARG, and (e) CEBPA in SCIDs and SHEDs after adipogenic induction. Student’s -test was performed to determine statistical significance. All error bars represent s.d. (). *. **. NS: no significant difference.
