Comparative Analysis of Proliferation and Differentiation Potentials of Stem Cells from Inflamed Pulp of Deciduous Teeth and Stem Cells from Exfoliated Deciduous Teeth
Figure 4
SCIDs and SHEDs showed similar chondrogenic differentiation potential, but SCIDs secreted more TNF-α protein than SHEDs. (a, b) Both SCIDs and SHEDs were cultured with chondrogenic differentiation medium for 3 weeks (21 d). Alcian blue staining revealed that proteoglycan production increased similarly in SCIDs and SHEDs. (c) Real-time RT-PCR results show significantly enhanced expression of COL2 in SCIDs and SHEDs after chondrogenic induction. (d) SOX9 mRNA detected with real-time RT-PCR in SHEDs and SCIDs before (0 d) and after (14 d) chondrogenic induction. Student’s -test was performed to determine statistical significance. (e) Real-time RT-PCR results show IL-1β, IL-6, and TNF-α expression. The expression levels were not significantly different between SCIDs and SHEDs. (f) IL-6 and TNF-α secretion levels were measured by ELISA. TNF-α secretion was significantly enhanced in SCIDs compared with SHEDs. IL-6 secretion was not different between SCIDs and SHEDs. All error bars represent s.d. (). *. **. NS: no significant difference.
