Structural Basis of Binding and Rationale for the Potent Urease Inhibitory Activity of Biscoumarins
Figure 2
Steady state inhibition of J.B. urease by compound 7; (a) is the Lineweaver-Burk plot of reciprocal of initial velocities versus reciprocal of four fixed urease concentrations in absence (■) and presence of 12.5 µM (□), 25.0 µM (●), and 50 µM (○) of compound 7. (b) is the Dixon plot of reciprocal of the initial velocities versus various concentrations of compound 7 at fixed urease concentrations, (■) 50 µM, (□) 25.0 µM, (●) 12.5 µM, and (○) 6.2 µM; (c) is the, versus various concentrations of compound 1. (d) is the, 1/Slope versus various concentrations of compound 7. (e) is the Lineweaver-Burk plot of reciprocal of initial velocities versus reciprocal of four fixed B.P. urease concentrations in absence (□) and presence of 12.5 µM (□), 25.0 µM (■), and 50 µM (▼) of compound 2. (f) is the Dixon plot of reciprocal of the initial velocities versus various concentrations of compound 2 at fixed urease concentrations, (○) 50 µM, (□) 25.0 µM, (■) 12.5 µM, and (▼) 6.2 µM. (g) is the versus various concentrations of compound 2. (h) is the, 1/Slope versus various concentrations of compound 2. (i) is the Lineweaver-Burk plot of reciprocal of initial velocities versus reciprocal of four fixed B.P. urease concentrations in absence (○) and presence of 12.5 µM (□), 25.0 µM (■), and 50 µM (∆) of compound 3. (j) is the Dixon plot of reciprocal of the initial velocities versus various concentrations of compound 3 at fixed urease concentrations, (■) 50 µM, (□) 25.0 µM, (▼) 12.5 µM, (▼) 6.2 µM, and (○) 3.1 µM. K is the versus various concentrations of compound 3. l is the 1/Slope versus various concentrations of compound 3.
