Chromatographic Fingerprint Analysis and Effects of the Medicinal Plant Species Mitracarpus frigidus on Adult Schistosoma mansoni Worms
Figure 3
HPLC chromatogram of Mitracarpus frigidus methanolic extract (MFM). The analysis was performed using a linear gradient of a binary solvent system A (water pH adjusted to 4.0 with H3PO4) : B (acetonitrile). The elution conditions applied were 0–20 min, 5–80% B and 20–30 min, 80–95% B. It was run at a flow rate of 1 mL/min over 30 minutes, with an injection volume (“loop”) of 20 μL and UV detection was at 230 nm.
