Upregulation of Pluripotency Markers in Adipose Tissue-Derived Stem Cells by miR-302 and Leukemia Inhibitory Factor
Figure 1
(a) Third-passaged ADSCs with a homogenous fibroblast-like morphology, (b) expression of stem cell marker, Sca-1, mesenchymal stem cell markers, CD73 and CD105, and hematopoietic cell markers, CD34 and c-Kit in the freshly isolated (ADSC P0) and third-passaged ADSCs (ADSC P3), (c) flow cytometry histograms of third-passaged ADSCs for hematopoietic (CD45 and CD11b), endothelial (CD31), and mesenchymal stem cell (CD29, and CD105) markers, and (d) expression of three germ layer markers, AFP, Pax6, and Brachyury, in the freshly isolated (ADSC P0) and third-passaged ADSCs (ADSC P3).
