Upregulation of Pluripotency Markers in Adipose Tissue-Derived Stem Cells by miR-302 and Leukemia Inhibitory Factor
Figure 3
(a) Immunostaining of the freshly isolated ADSCs (ADSC P0) with anti-OCT3/4 antibody, (b) propidium iodide (PI) staining of the nuclei represented in (a). (c–e) Phase contrast, PI staining, and OCT3/4 immunostaining of the ADSC P0. Immunostaining was performed using a specific monoclonal antibody (sc-5279) against the OCT4A protein. Nuclear signal was detected in the ADSCs and recognized the OCT4A isoform. (f) Immunostaining of third-passaged ADSCs (ADSC P3) with anti-OCT3/4 antibody, (g) PI staining of the nuclei represented in (f).
