Upregulation of Pluripotency Markers in Adipose Tissue-Derived Stem Cells by miR-302 and Leukemia Inhibitory Factor
Figure 7
(a and b) Expression of EGFP in the third-passaged ADSCs, after three-day antibiotic selection, (c) western blot analysis for the expression of OCT4A protein in the transfected and control ADSCs, after 5-day antibiotic selection, and (d) quantitative real-time RT-PCR analysis for the expression of OCT4, Nanog, Sox2, and AFP mRNAs in the miR-302 group comparing to mock group, 48 hours after transfection. Tubb5 mRNA level was used as an internal control. was considered as significant.
