Upregulation of Pluripotency Markers in Adipose Tissue-Derived Stem Cells by miR-302 and Leukemia Inhibitory Factor

Table 1

Primers used for RT-PCR and qPCR.

Genes	Forward	Reverse	Size (bp)	Accession number
Sca-1	5′-CTCTGAGGATGGACACTTCT-3′	5′-GGTCTGCAGGAGGACTGAGC-3′	404	NM_001271416
CD34	5′-ATGCAGGTCCACAGGGACACG-3′	5′-CTGTCCTGATAGATCAAGTAG-3′	220	NM_001111059
c-kit	5′-CCATGTGGCTAAAGATGAAC-3′	5′-ACTGCTGGTGCTCGGGTTT-3′	318	NM_001122733
CD73	5′-TCCTGGGCTACGATGCTATG-3′	5′-CCACAACCTCACCGCCAAC-3′	195	NM_011851
CD105	5′-AGCCTTACCTCTGGATACCG-3′	5′-AACGTCACCTCACCCCTTGT-3′	190	NM_007932
OCT4A	5′-TGTGGACCTCAGGTTGGACT-3′	5′-CTTCTGCAGGGCTTTCATGT-3′	201	NM_013633
Sox-2	5′-GCACATGAACGGCTGGAGCAACG-3′	5′-TGCTGCGAGTAGGACATGCTGTAGG-3′	206	NM_011443
Nanog	5′-GAGTGTGGGTCTTCCTGGTC-3′	5′-GAGGCAGGTCTTCAGAGGAA-3′	182	NM_028016
Pax6	5′-TGCCCTTCCATCTTTGCTTG-3′	5′-TCTGCCCGTTCAACATCCTTAG-3′	178	NM_001244200
Brachyury	5′-ATGCCAAAGAAAGAAACGAC-3′	5′-AGAGGCTGTAGAACATGATT-3′	835	NM_009309
AFP	5′-TCGTATTCCAACAGGAGG-3′	5′-AGGCTTTTGCTTCACCAG-3′	174	NM_007423
Tubb5	5′-GGAACATAGCCGTAAACTGC-3′	5′-TCACTGTGCCTGAACTTACC-3′	317	NM_011655