Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells In Vitro
Figure 4
Effect of compounds 1 and 2 on HSV-1 propagation. (a) PC-12, (b) Neuro-2a, and (c) SH-SY5Y cells monolayers grown in coverslips inside 24-well plates were infected with HSV-1 Cgal and incubated for 1 h at 37°C. Then, cells were covered with DMEM/F12 2% or media containing 10 μM of compound 1 and 5 μM of compound 2. After incubation for 24 h, cells were stained in situ for β-gal. Cgal: HSV-1 Cgal infected-untreated cells; Cgal+1: HSV-1 Cgal infected 1-treated cells; Cgal+2: HSV-1 Cgal infected 2-treated cells. Magnification: 40x.
