Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells In Vitro
Figure 5
Effect of compounds 1 and 2 on HSV-1 KOS multiplication in three nervous cell lines. Neuro-2a cells grown in coverslips inside 24-well plates were infected with HSV-1 KOS wt and incubated for 1 h at 37°C. Then, cells were covered with DMEM/F12 2% or media containing 10 μM of compound 1 and 5 μM of compound 2. After 24 h p.i., cells were fixed with methanol and HSV-1 gD localization was done by IFI staining. (a) Control cells; (b) mock-infected cells treated with compound 1, (c) mock-infected cells treated with compound 2, (d) HSV-1 KOS infected cells, (e) HSV-1 KOS infected cells treated with compound 1, and (f) HSV-1 KOS infected cells treated with compound 2. Magnification: 40x.
