[Retracted] Immunomodulation in Human Dendritic Cells Leads to Induction of Interferon-Gamma Production by Leishmania donovani Derived KMP-11 Antigen via Activation of NF-κB in Indian Kala-Azar Patients

Figure 3

(a) IL-12 release by MΦ and moDC after KMP-11 and PHA stimulation. (a) Comparison of cytokine production by APCs after costimulation by KMP-11. 1 × 10⁶/mL of either CD14+ MΦ or CD83+ moDCs was stimulated with KMP-11 (10 μg/mL) and PHA (10 μg) for 16 h. Harvested cells were consecutively coincubated with Bref-A (1 μg/mL), surface CD-4 (PE)/CD-83 (PE) antibodies, and cytofix/Perm solution before cytoplasmic staining with FITC for IL-12 and acquired and analyzed on FACS-calibur. Values were expressed in mean ± SEM. Each sample was run in duplicate. ^∗∗∗𝑃<0.001 versus VL-MΦ; ^###𝑃<0.001 versus VL-DC; ^∧∧∧𝑃<0.001 versus Ctrl-DC; ^@𝑃<0.05 versus VL-MΦ-KMP-11 and ^$$$𝑃<0.001 versus respective MΦ and DC groups; and ⁺𝑃<0.05 between VL-DC-KMP-11 and Ctrl-DC-KMP-11 groups. (b) Representative dot plot for the intracellular staining of IL-12 in MΦs and DCs of VL patients.

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