Perinatal Nitric Oxide Therapy Prevents Adverse Effects of Perinatal Hypoxia on the Adult Pulmonary Circulation

Figure 2

Pharmacological response of isolated pulmonary arteries to the endothelium-dependent agent acetylcholine. Dose-response to acetylcholine (ACh) was tested in pulmonary arteries preconstricted with phenylephrine (10⁻⁵ M), isolated from adult mice born in normoxia (Ctr), in hypoxia (PH), or in hypoxia with simultaneous administration of iNO (PH + NO). Results are expressed as mean ± SEM of percentage of change in tension induced by the vasodilator (𝑛=6--9 mice). ∗Significant difference compared to controls and ^†significant difference between mice exposed to perinatal hypoxia and iNO and mice with perinatal hypoxia alone (𝑃<0.05, two-way ANOVA). Results obtained with pulmonary arteries of mice born in normoxia (Ctr) or in hypoxia (PH) were previously published [13].