Biochemical and Functional Characterization of Parawixia bistriata Spider Venom with Potential Proteolytic and Larvicidal Activities
Figure 1
Proteolytic activity of Parawixia bistriata crude venom on Fibrogen. Fibrinogen was preincubated with the crude venom of P. bistriata in varying concentrations, times, temperatures, and pHs. The samples were subjected to SDS-PAGE for visualization of protein bands. Staining: Coomassie Blue.F = Fibrogen CV = P. bistriata crude venom. (a) Effect of concentration. Lanes: (a)1: F (80 μg); (a)2: F + CV (10 μg); (a)3: F + CV (20 μg); (a)4: F + CV (40 μg); (a)5: F + CV (60 μg); (a)6: F + CV (80 μg); (a)7: F + CV (100 μg); (a)8: CV (50 μg). (b) Effect of incubation time. Lanes: (b)1: F (80 μg); (b)2: F + 20 μg CV (30′); (b)3: F + 20 μg CV (1 h); (b)4: F + CV (2 h); (b)5: F + CV (3 h); (b)6: F + CV (6 h); (b)7: F + CV (12 h); (b)8: F + CV (24 h). (c) Effects of different temperatures. Samples: (c)1: F (80 μg); (c)2: F + CV (20 μg) (−10°C); (c)3: F + CV (4°C); (c)4: F + CV (25°C); (c)5: F + CV (37°C); (c)6: F + CV (50°C); (c)7: F + CV (70°C); (c)8: F + CV (100°C). (d) Effects of different pHs. Samples: (d)1: F (80 μg); (d)2: F + CV (20 μg) (pH 2.5); (d)3: F + CV (pH 3.5); (d)4: F + CV (pH 4.5); (d)5: F + CV (pH 5.5); (d)6: F + CV (pH 7.0); (d)7: F + CV (pH 8.0); (d)8: F + CV (pH 9.0); (d)9: F + CV (pH 10.0).
