Anti-Inflammatory Effect and Mechanism of the Green Fruit Extract of Solanum integrifolium Poir.
Figure 2
The methanol fraction of Solanum integrifolium (ME) inhibits LPS-induced iNOS and COX-2 expression in RAW264.7 macrophages. (a and d) RAW264.7 macrophages were cultured with vehicle (0.1% DMSO) or LPS (10 ng/mL) in the presence of the indicated concentrations of ME in 6-well plates for 16 h. Total cell lysates were prepared and the iNOS and COX-2 protein expressions were detected by Western blotting, as described in Section 2. The levels of α-tubulin in the total lysates serve as loading control. The blot is representative of at least three experiments. (b and c) RAW264.7 cells were cultured as described above for 12 h. Total RNA was prepared and the mRNA levels of iNOS and COX-2 were quantified by RT-Q-PCR, as described in Section 2. Data represent the mean ± SD of three independent experiments. represents significant differences compared with the vehicle control (without LPS). ; represent significant differences compared with the LPS-treated vehicle.
